In this bulletin we highlight Why, When & How to use direct amplification of the 16S rRNA gene for bacterial identification. Broad-range amplification of the bacterial 16S gene directly from clinical samples followed by sequencing is particularly valuable when:
- A patient has received antibiotics prior to specimen collection
- A clinician has a high index of suspicion for an infection caused by atypical or anaerobic bacteria
- The infection may be caused by multiple bacteria
The bulletin highlights general considerations and can serve as information material for clinicians.
Useful references display the utility of direct detection.