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In Vivo modell för evaluering av penetration vid laser behandling

Ofta får vi frågan om penetrationsdjup mellan klass4, 3B och 2... Kliniska studier visar på en kaskad effekt i cellerna vid behandling med 405nm - 635nm fotobiokemisk laser (synliga spektrumet av ljus). Orsaken till detta är den eV som fotonpartiklarna bär inom detta spektrum, vilket startar elektrontransportkedjan vilket leder till ökad ATP. Less is more är en bra tanke att ha med sig.

Abstract

Photobiomodulation therapy (PBMT) effects depend on the energy settings and laser penetration. We investigated the penetration time profiles of two different light therapy devices, at the dark and light skin regions in horses. Six light skin and six dark skin adult clinically healthy Arab and Quarter horses were used. A cutometer was used to measure the width of the skin fold from both sides of the cervical area, followed by three measurements of the thickness of the same skin fold by transversal and longitudinal ultrasonography (US). The depth of light penetration was compared based on the percentage of penetration versus power, between a portable PBMT device versus a class IV laser device. The laser mean power output was measured with an optical power meter system for 120 seconds after penetrating the skin. Skin width and laser penetration were compared among equipment by paired “t” test. There was no difference in the width of the skin fold between measurements acquired by the cutometer against either longitudinal or transversal US or between the US measurements at cervical versus metacarpus area. Light penetration was greater in both kinds of skins in the PBMT (0.01303 ± 0.00778) versus class IV laser (0.00122 ± SD 0.00070) (P < .001). The PBMT device provided a greater energy penetration than the class IV laser in unclipped light and dark skin, suggesting that the former may produce a better therapeutic effect. The color of the skin changes penetration profiles of PBMT.

School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (Unesp), Botucatu, São Paulo, BrazilbCenter for Integrative Animal Health, Salt Spring Island, British Columbia, Canada

Received 13 September 2019, Revised 5 November 2019, Accepted 8 November 2019, Available online 4 December 2019.

Animal welfare/ethical statement: The authors certify that legal and ethical requirements have been met with regard to the humane treatment of animals according to the legislation of the Brazilian Council of Control in Animal Experimentation (http://www.mctic.gov.br/mctic/opencms/institucional/concea/index.html). The study was approved by the Animal Research Ethical Committee from the School of Veterinary Medicine and Animal Science, University of São Paulo State, under the protocol number 0103/2018.

Conflict of interest statement: A. Schoen is an occasional consultant for Multi Radiance Medical (Solon, OH), a laser device manufacturer, but did not participate in data acquisition, management, and interpretation of data or statistical analysis. Other authors do not have conflict of interest. Data storage and documentation: data are available on request.

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Arch of Scandinavia agerar för en intelligent och effektiv hälsovård med en strävan att ersätta viss medicin med validerad, kliniskt bevisad frekvens-medicinsk vård, utan biverkningar. Förutom den skada över nyttjandet av kemiska preparat orsakar människor och djur, kan man idag inte bortse från den miljömässiga påverkan som medicintillverkning/konsumtion har i form av utsläpp av stabila kemiska sammansättningar i marker och vattendrag, ca 4kg/1Milj liter vatten. #skyddaantibiotikan

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