Toll-like receptor 4 (TLR4) plays a fundamental role in the activation of the innate immune response, which is why drug development of TLR4 antagonists is a hot topic for treatment of inflammatory diseases such as inflammatory bowel disease (IBD), rheumatoid arthritis and multiple sclerosis.
Euro Diagnostica is proud to presents a new, highly specific and sensitive reporter gene cell line, responsive to stimulation of TLR4.
The iLite™ TLR4 cell line is launched as Assay Ready Cells, which can be utilized for determining functional activity of potential TLR4 antagonists and agonists. The cells express Firefly Luciferase under the control of a TLR4 responsive promotor and when stimulating the cells with e.g. LPS, the resulting luminescent signal is proportional to the functional activation of TLR4.
Conversely, in an inhibition assay, the cells can be utilized for determining a TLR4 antagonist's ability to block the signaling and activation of TLR4.
Contact us today to find out more about iLite™ Assay Ready Cells!