2011-07-14 08:24 Wiklander et al: A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of radioligand binding studies and surface area analyses demonstrated the presence of selectivity in polymers prepared using methacrylic acid as the functional monomer. See link for full abstract! -->

2011-07-06 10:25 Dahlbäck et al: During pregnancy, the interaction between malaria infected erythrocytes and the placental receptor is mediated by the parasite expressed protein VAR2CSA. A vaccine against placental malaria should induce antibodies inhibiting the interaction between VAR2CSA and CSA. In this study, we used a biosensor technology to examine binding properties... see link for full article! -->

2011-06-28 17:05 Lonza and Attana in collaboration: The combination of computational and protein engineering tools with novel orthogonal methods of measuring aggregation can be used to assess the developability of biopharm products in the early stages of development, thereby reducing the level of risk in later stages.

2011-07-11 08:59 Lund University: Molecularly imprinted polymers (MIPs) were prepared in situ on Attana's quartz crystal sensor surface with good reproducibility and long-term stability. The resulting MIP surfaces can be used for detection and discrimination between very similar molecules, and the analysis is fast enough to allow for large amounts of samples.

2011-06-22 08:44 University of California, San Fransisco (UCSF): A method has been optimized to extract Botulinum toxin A from the inclusion body, refold it into active form and purify it using ion exchange chromatography. A rapid immunoassay was also developed to facilitate monitoring of active Botulinum toxin A during purification, using Attana's quartz crystal microbalance sensor technology.

2011-06-20 08:28 Two experiments with two distinct purposes were performed on the Attana Cell 200. The first experiment intended to find a diagnostic tool to test tumors after surgery to see if they are metastatic. The second experiment tested a herceptin conjugate designed for targeted immunotherapy. Both results showed that the Attana Cell 200 may help lowering clinical stage failure rates.

2011-06-15 08:25 Massachusetts Institute of Technology (MIT): A biocompatible and injectable designer self-assembling peptide hydrogel system has been developed. It may be a useful carrier for the presentation of functional proteins and therapeutic antibodies for sustained release applications.

2011-06-13 08:43 Polypure: By using functionalized PEG derivatives, gold particles can be modified or conjugated to biomolecules to increase the stability. Avidin has been used as a tetrameric multivalent "glue" to demonstrate cluster formation between biotinylated AuNPEGs (Polyethylene glycol coated gold nanoparticles).

2011-06-08 08:31 Swiss Institute of Allergy and Asthma Research (SIAF): Currently we are using QCM to replace classical surface plasmon resonance experiments to quantify molecular interactions. We aim to establish this novel technology of measuring the interaction of biomolecules with molecules on entire cells, and to elucidate the binding properties of these interactions on defined systems.

2011-06-06 08:42 Lonza: This poster shows how the Lonza Biologics AggreSolve platform has been expanded and successfully applied to engineer humanized antibodies with good developability profiles; such antibodies have good stability, productivity and retain activity of therapeutic levels, eliminating the need for further, potentially destabilizing, affinity maturation.

2011-05-27 09:12 Attana and Layerlab: Lipoparticles containing a CXCR4 chemokine receptor were immobilized using memLAYER on the Attana LNB Carboxyl chip. The applicability of lipoparticles and memLAYER for functional studies of GPCRs on the Attana A200 biosensor are demonstrated as well as the benefits of the memLAYER-immobilization.

2011-05-30 09:05 University of Stuttgart, Institute of Cell Biology and Immunology: A humanized IgG1 version (ATROSAB) of a murine TNFR1-selective antagonistic antibody (H398) has been generated. The ATROSAB shows identical binding characteristics and sub-nanomolar affinity to both human and rhesus TNFR1.

2011-06-01 09:03 To facilitate the study of carbohydrate-protein interactions a method for label-free measurements has been developed using the Attana 100 biosensor to monitor binding events in real time. The method relies on measurement of the inhibition of a well-known carbohydrate-lectin binding pair, Concanavalin A - Yeast mannan, by lectin binding carbohydrates.

2011-04-13 08:58 Attana has several Quartz Crystal Microbalance (QCM) based instruments and applications, all catering to crucial needs and developed in close collaboration with industry and universities. Examples include kinetic analysis on cell surfaces, candidate selection screening, aggregation characterization, epitope mapping and concentration determination of biopharmaceuticals.

2011-01-26 08:53 The journal IPT features an article written by Attana's product manager where he discusses how to achieve biological relevance during research and the benefits of doing so. The article is also available online, see right for link.

2010-11-11 09:02 Kinetics on cells using the Attana Cell 200 - two application examples easily explained in Attana's new poster. Select the right candidate early by getting biologically relevant data from interactions directly on the cell surface.

2010-06-23 08:46 The objective of this application note was to determine the binding properties of the lectin ConA towards human epidermoid carcinoma cells. Among other things it was found that a kinetic approach may be used to determine the apparent affinity and rate constants of lectin-cell glycan interactions, providing a greater understanding of the mechanism involved in the lectin recognition of cancer cells.

2010-06-16 09:42 The objective of this application note was to evaluate newly developed therapeutic antibodies in a biologically relevant context (cells) in order to detect failing candidates at an earlier stage. Among other things, it was found that real-time screening of drug candidates in a synthetic and in a cellular context together provides highly informative and biologically relevant data.

2010-06-09 08:34 The objective of this application note was to determine the glycosylation profile of cancer cells using a cell based QCM Biosensor assay. Among other things, it was found that a single surface can be used to perform lectin screening and to provide a highly relevant and reproducible determination of the glyco-profile of cancer cells.