Apr 04, 2012 10:28 EDT Three different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. Read the full abstract here: www.attana.com/pub_antibodies_proteins

Mar 28, 2012 10:22 EDT The photoinitiated radical reactions between thiols and alkenes/alkynes (thiol-ene and thiol-yne chemistry) have been applied to a functionalization methodology to produce carbohydrate-presenting surfaces for analyses of biomolecular interactions. Read the full abstract here: www.attana.com/pub_carbohydrates

Mar 20, 2012 11:10 EDT The real-time lectin screening of a range of lectins, including ConA, DBA, PNA and UEA-I, enabled the accurate study of the glycosylation changes between cells, such as changes associated with cancer progression and development. Furthermore, the kinetic parameters of the interaction of Con A with MDA-MB-468 cells were studied. Read the full abstract here: www.attana.com/pub_cells

Mar 06, 2012 09:05 EST Single-walled carbon have properties that make them useful for drug delivery, and their photo-thermal effects make them potentially useful in a wide range of applications, particularly the treatment of solid tumors. Follow link for full abstract: www.attana.com/pub_cells. See abstract on video (Attana Cell 200 data shown in min 04:55) http://www.dovepress.com/articles.php?article_id=9317

Jul 11, 2011 09:54 EDT Lund University: Molecularly imprinted polymers (MIPs) were prepared in situ on Attana's quartz crystal sensor surface with good reproducibility and long-term stability. The resulting MIP surfaces can be used for detection and discrimination between very similar molecules, and the analysis is fast enough to allow for large amounts of samples.

Jun 22, 2011 09:50 EDT University of California, San Fransisco (UCSF): A method has been optimized to extract Botulinum toxin A from the inclusion body, refold it into active form and purify it using ion exchange chromatography. A rapid immunoassay was also developed to facilitate monitoring of active Botulinum toxin A during purification, using Attana's quartz crystal microbalance sensor technology.

Jun 20, 2011 10:32 EDT Two experiments with two distinct purposes were performed on the Attana Cell 200. The first experiment intended to find a diagnostic tool to test tumors after surgery to see if they are metastatic. The second experiment tested a herceptin conjugate designed for targeted immunotherapy. Both results showed that the Attana Cell 200 may help lowering clinical stage failure rates.

Jun 13, 2011 10:34 EDT Polypure: By using functionalized PEG derivatives, gold particles can be modified or conjugated to biomolecules to increase the stability. Avidin has been used as a tetrameric multivalent "glue" to demonstrate cluster formation between biotinylated AuNPEGs (Polyethylene glycol coated gold nanoparticles).

Jun 08, 2011 09:24 EDT Swiss Institute of Allergy and Asthma Research (SIAF): Currently we are using QCM to replace classical surface plasmon resonance experiments to quantify molecular interactions. We aim to establish this novel technology of measuring the interaction of biomolecules with molecules on entire cells, and to elucidate the binding properties of these interactions on defined systems.

Jun 06, 2011 09:53 EDT Lonza: This poster shows how the Lonza Biologics AggreSolve platform has been expanded and successfully applied to engineer humanized antibodies with good developability profiles; such antibodies have good stability, productivity and retain activity of therapeutic levels, eliminating the need for further, potentially destabilizing, affinity maturation.

May 30, 2011 10:29 EDT University of Stuttgart, Institute of Cell Biology and Immunology: A humanized IgG1 version (ATROSAB) of a murine TNFR1-selective antagonistic antibody (H398) has been generated. The ATROSAB shows identical binding characteristics and sub-nanomolar affinity to both human and rhesus TNFR1.

May 27, 2011 03:26 EDT Attana and Layerlab: Lipoparticles containing a CXCR4 chemokine receptor were immobilized using memLAYER on the Attana LNB Carboxyl chip. The applicability of lipoparticles and memLAYER for functional studies of GPCRs on the Attana A200 biosensor are demonstrated as well as the benefits of the memLAYER-immobilization.

Jun 01, 2011 03:20 EDT To facilitate the study of carbohydrate-protein interactions a method for label-free measurements has been developed using the Attana 100 biosensor to monitor binding events in real time. The method relies on measurement of the inhibition of a well-known carbohydrate-lectin binding pair, Concanavalin A - Yeast mannan, by lectin binding carbohydrates.

Apr 15, 2011 04:07 EDT The data obtained from QCM analyses are firstly qualitative, delivering information about specificity and selectivity, and quantitative, delivering exact thermodynamic and kinetic parameters of interactions. See the difference in this poster!

Apr 07, 2011 04:17 EDT Attana has several Quartz Crystal Microbalance (QCM) based instruments and applications, all catering to crucial needs and developed in close collaboration with industry and universities. Examples include kinetic analysis on cell surfaces, candidate selection screening, aggregation characterization, epitope mapping and concentration determination of biopharmaceuticals.