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COMplementary Primer ASymmetric PCR (COMPAS-PCR) Applied to the Identification of Salmo salar, Salmo trutta and Their Hybrids

Dokument   •   mar 31, 2017 16:05 CEST

We show that 5’-complementarity between primers may be exploited in a new PCR method called COMplementary-Primer-Asymmetric (COMPAS)-PCR, using asymmetric primer concentrations to achieve target PCR amplification. This small paradigm shift, using highly complementary primers for PCR, should help develop robust assays that previously would not be considered.
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