HAMILTON NIMBUS IN THE SECRETOME WORKFLOW

At the group belonging to Professor Mathias Uhlen, the Protein Factory at KTH Royal Institute of Technology in Stockholm, have since the last year been using the Hamilton Microlab Nimbus. One of the users, Henric Enstedt explains:

"In the Wallenberg Center for Protein Research at KTH Royal Institute of Technology we aim to produce, purify and analyse all the human secreted proteins. In order to do this, we produce proteins in CHO cells and purify those using a standardized high throughput system. After the proteins have been successfully purified, they are desalted using spin columns. The loading of proteins onto the columns is neatly done using the 4 channel Nimbus. This frees up time and ensures that the procedure is done in exactly the same way every time."

The simplicity in the software has allowed to group to add more methods to their workflow. Henric continues:

"After desalting, the samples are aliquoted to our different partners and for internal use. Our LIMS system calculates how much of the proteins should go to different aliquots depending on source volume and concentration. It then generates a worklist that controls the robot during the run, by specifying which channels and tips to use, volumes to be pipetted and when to change tips. By having thus structure, we can change the aliquotation strategy without needing to change the method controlling the robot." 

Shortly: the LIMS system and the Hamilton Microlab Nimbus is collaborating and controlling all aspects of the Nimbus. From what plate type, which position, volume an aspiration should occur as well as to which plate type, position and volume to dispensing should occur. The staff only have to place the correct plates at the correct position - the LIMS and the Nimbus will take care of the rest - Full process safety and process control. 

If you are interested how Hamilton can help you with your liquid handling challenges please contact us at info.nordic@hamilton.ch. Please also look at the attached PDF for more pictures and information about the Microlab Nimbus from Hamilton.