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Comparing Protein A Resins for Monoclonal Antibody Purification

News   •   Dec 17, 2013 14:47 GMT

Source: Biopharm International

Dec 1, 2013 
By: Shohei KobayashiYasufumi Ueda 
BioPharm International Volume 12, Issue 26, pp. 28-31 

The methodology for the production of monoclonal antibodies from a cell line by hybridization of mouse myeloma and mouse spleen cells from an immunized donor was first published in 1975 (1). As a technology that permits the generation of monoclonal antibodies against almost any target molecule, it immediately gained great interest as a source for potential drug candidates. Although it took some time for the first therapeutic mAb to become commercially available in 1986 (2), the market for therapeutic mAbs has since grown rapidly. MAbs have proved to be successful as targeted therapeutics for a variety of diseases, including several forms of cancer, multiple sclerosis, and immunological disorders such as rheumatoid arthritis and psoriasis. In 2007, mAbs accounted for almost half of the top-20 best-selling biotechnology drugs in the US alone, establishing them as an important group of molecules (3). Today, mAbs constitute the single largest class of biological drugs and accounts for about 36% of the total biologics market with an annual sales growth rate of approximately 10% (4).With greater economic pressure on monoclonal antibody (mAb) production for therapeutic and research uses, antibody titers in mammalian cell culture have increased dramatically over the past 20 years. As a consequence, downstream processing must accept and handle higher titers of mAbs in harvested cell-culture fluid (HCCF), and vendors of mAb purification technologies must develop chromatography resins with high binding capacity to meet the demand. In addition, more cost-efficient cleaning procedures are necessary to extend the lifetime of chromatography resins and to reduce the cost for cleaning and validation. A team from Chugai Pharmaceutical (Japan) investigated the mAb purification performance of a new alkali-tolerant, prototype Protein A resin (Resin 3, MabSelect SuRe LX prototype, GE Healthcare), which has the potential to address the demand for a more advanced, cost-effective mAb purification technology. 

Commercial-scale purification of mAbs usually contains two or three chromatographic steps. Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Protein A-based resins form the basis of almost all mAb-purification platforms as they are easy to use at both small and large scale with generic experimental protocols.Commercial-scale production challenges
The rapid growth in mAb demand has triggered industry efforts to increase manufacturing capacity, with the consequence that the antibody titers in mammalian cell culture have increased dramatically. Today, a typical process accumulates titers of 1-5 g/L, but expression levels as high as 10-13 g/L have been reported (5). The increase in upstream productivity creates a subsequent demand on downstream processing to address high-titer HCCF.

Increased antibody titers create a potential purification challenge because of the limited capacity of current Protein A resins. To handle the high titers, new resins with significantly greater capacity are needed. In addition, Protein A resins with the ability to withstand repeated cleaning-in-place (CIP) with low-cost sodium hydroxide (NaOH) considerably improves process economics. 

Protein A Resins
Protein A is a bacterial protein from Staphylococcus aureus, with the capacity to bind mammalian antibodies of class immunoglobulin G (IgG) with high affinity. The gene for Protein A has been cloned and expressed in Escherichia coli(6, 7) allowing for the production of large quantities of recombinant Protein A.

Although recombinant Protein A is widely used as an affinity ligand for the capture and purification of antibodies, its sensitivity to alkaline conditions prevents the use of rigorous and cost-effective CIP and sanitization protocols based on NaOH.

Compared to conventional Protein A resins, one of the affinity chromatography resins investigated, Resin 2 (MabSelect SuRe, GE Healthcare) is based on a modified alkali-tolerant Protein A ligand. Through protein engineering, the amino acids in one of the IgG-binding domains particularly sensitive to alkali were identified and substituted with more stable ones.

A novel prototype Resin 3 offers an increased dynamic binding capacity (DBC) at a slightly longer residence time.  

Evaluating capacity and reusability
Table I
 shows the Protein A resins that were compared for performance and cost-efficiency. According to the vendor, Resin 3 exhibits higher DBC than Resin 2 at longer residence times (8). A residence time of 6 min is expected to give a DBC (at 10% breakthrough) of approximately 60 g antibody per liter resin. In this study, the authors were able to confirm this behavior, and for further studies a residence time of 6 min and a loading of 50 g antibody per liter resin, corresponding to approximately 80% of the DBC (at 10% breakthrough), were selected.

Table I. Properties of Protein A resins; degree of alkali resistance is indicated by +/-.

Resin number


Average particle size (µm)

Alkali resistance


Binding capacity*

Resin 1 (rProteinA Sepharose 4 Fast Flow)

rProtein A




~27 g/L resin

Resin 2 (Mab Select SuRe)

Alkali-tolerant Protein A




~35 g/L resin

Resin 3 (Mab Select SuRe LX prototype)

Alkali-tolerant Protein A




~60 g/L resin

* Typical dynamic binding capacities according to resin manufacturer data

Table II presents the outline of the lifetime study. An amount of cell-culture supernatant corresponding to 50-g antibody per liter resin was applied to the column at 6 min. residence time. This was followed by a two-step washing procedure to remove unbound particles. Bound antibody was eluted with 50 mM acetic acid and the column resin was cleaned in place with five column volumes (CV) of 0.1 M NaOH.

Table II. Study outline. Column height = 100 mm and inner diameter = 10 mm, run time = 5 h, CV is column volume.




Flow rate


20 mM citrate-phosphate buffer 
pH 7.5, 1 mol/L sodium chloride (NaCl)

5 CV

300 cm/h


Harvested cell-culture fluid

50 g/L resin 
(residence time 6 min)

100 cm/h

Wash 1

20 mM citrate-phosphate buffer 
pH 7.5, 1 mol/L NaCl

5 CV

300 cm/h

Wash 2

10 mM citrate-phosphate buffer 
pH 7.7

5 CV

300 cm/h


50 mM acetic acid

6 CV

300 cm/h


0.1 mol sodium hydroxide

3 CV

120 cm/h

Storage (per 4 cycles)

2% benzyl alcohol, 50 mM sodium acetate, pH 5.0

5 CV

120 cm/h

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