COMplementary Primer ASymmetric PCR (COMPAS-PCR) Applied to the Identification of Salmo salar, Salmo trutta and Their Hybrids

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COMplementary Primer ASymmetric PCR (COMPAS-PCR) Applied to the Identification of Salmo salar, Salmo trutta and Their Hybrids

We show that 5’-complementarity between primers may be exploited in a new PCR method called COMplementary-Primer-Asymmetric (COMPAS)-PCR, using asymmetric primer concentrations to achieve target PCR amplification. This small paradigm shift, using highly complementary primers for PCR, should help develop robust assays that previously would not be considered.
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Emner

  • Nye publikasjoner

Kategorier

  • repeated sequences
  • forskning
  • research
  • dna
  • pcr
  • niva

Regions

  • Oslo

Kontakter

Gunnar Omsted

Pressekontakt Seniorrådgiver, Medier og formidling Pressekontakt 93654386

Caroline Enge

Pressekontakt Forskningsassistent, seksjon for vann og samfunn Kommunikasjon, forskning +4745447990

Relatert innhold

A new approach to amplifying DNA; A small paradigm shift within molecular photocopying

Methods used for DNA-analyses often require more DNA than may be available in a typical sample. ‘Therefor, amplification is necessary, but not always straightforward. The most widely used amplification or photocopying method is the polymerase chain reaction (PCR). A new PCR method could help the amplification process, and thus develop robust assays that previously would not have been possible.